L-4-Hydroxy Proline

Synonyms

(2S,4R)-4-Hydroxypyrrolidine-2-carboxylic acid, Hyp, trans-4-Hydroxy-L-proline,
(2S,4R)-4-Hydroxyproline, L-hydroxyproline, hydroxyproline

Technical Data

CAS Number: 51-35-4
Molecular Formula: C₅H₉NO₃
Molecular Weight: 131.13
Beilstein Registry Number: 81441
EC Number: 200-091-9

FabriChem Specifications

ITEMS	SPECIFICATIONS
Description	White crystal or crystalline powder
Identification	Positive
Specific Rotation	-74° to -77°
pH	5.0 – 6.5
Chloride (Cl)	0.0020% Max.
Ammonium (NH₄)	0.020% Max.
Sulfate (SO₄)	0.020% Max.
Heavy Metals	10 ppm Max.
Iron (Fe)	10 ppm Max.
Arsenic (As₂O₃)	1 ppm Max.
Other Amino Acids	Conforms
Loss on Drying	0.20% Max.
Residue on Ignition	0.10% Max.
Assay	98.5 – 101.0%

Molecular Structure

l-4-hydroxy-proline-structure

About

Hydroxy Proline is a common non-proteinogenic amino acid. Hydroxy Proline is produced by hydroxylation of the amino acid proline by the enzyme prolyl hydroxylase following protein synthesis.

Hydroxy Proline is a major component of the protein collagen. Hydroxy Proline and proline play key roles for collagen stability. Hydroxy Proline is found in few proteins other than collagen. For this reason, Hydroxy Proline content has been used as an indicator to determine collagen and/or gelatin amount.