Guanidine Hydrochloride (High Purity)


aminoformamidine hydrochloride; aminomethanamidine hydrochloride;
guanidinium chloride; GdmCl; GndCl; GuHCl; hydrochloride salt of guanidine

Technical Data

CAS Number: 50-01-1
Molecular Formula: NH2C(=NH)NH2 · HCl
Molecular Weight: 95.53
Beilstein Registry Number: 3591990
EC Number: 200-002-3

FabriChem Specifications

Clarity & Color of SolutionClear, colorless
Ethyl Alcohol Solution (5%)Clear
Absorbance of Solution:
(6M, 20 ± 5° C)

At 230 nm
At 240 nm
At 260 nm

0.2 Max.
0.05 Max.
0.03 Max.
Loss on Drying1.0% Max.
Residue on Ignition0.05% Max.
Free-Acid0.01% Max.
Sulfate50 ppm Max.
NitratePasses Test
Iron2 ppm Max.
Lead0.5 ppm Max.
Copper0.2 ppm Max.
Arsenic0.5 ppm Max.
Total – CN5 ppm Max.
Free – CN0.1 ppm Max.
Purity99.5% Min.
Solubility (6 Mol/L in DIH2O)Soluble
Solubility (5 w/v% in Ethyl Alcohol)Soluble

Molecular Structure



Guanidine Hydrochloride is hydrochloride salt of guanidine. Guanidinium chloride is a strong chaotrope and one of the strongest denaturants used in physiochemical studies of protein folding. At high concentrations of guanidinium chloride, proteins lose their ordered structure, and they tend to become randomly coiled, i.e. they do not contain any residual structure. Guanidine Hydrochloride is often used at high concentrations in both RNA and protein purification procedures. It denatures native proteins and thus inactivates RNases and proteases. This compound is frequently used in RNA purification, particularly for the isolation of intact mRNA from tissues or cultured cells. It is also extremely useful for dissolving protein inclusion bodies.