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CHAPS

Synonyms

3-[dimethyl(3-{[(3α,5β,7α,12α)-3,7,12-trihydroxy-24-oxocholan-24-yl]amino}propyl)ammonio]-1-propanesulfonate,
3-[(3-cholamidopropyl)dimethylamino]-1-propanesulfonate

Technical Data

CAS Number: 75621-03-3
Molecular Formula: C32H58N2O7S
Molecular Weight: 614.88

FabriChem Specifications

ITEMSSPECIFICATIONS
Assay (from N)98% Min.
AppearanceWhite powder
Nitrogen (Kjeldahl)4.46% - 4.65%
Solubility (10% in H2O, 20˚C)Complete, colorless
Moisture2.0% Max.
TLC
(Silica gel:methanol/NH4OH = 95/5)
One Spot
Conductivity (0.1M in H2O)10 µs/cm Max.
pH (1% in H2O)5.0 – 8.0
Residue on Ignition0.2% Max.

Molecular Structure

chaps-structure

About

CHAPS {3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate} and CHAPSO [3-[(3-cholamidopropyl)dimethylammonio]-2-hydroxy-1-propanesulfonate] are zwitterionic (aka hybrid or dipolar) detergents used in the laboratory to solubilize biological macromolecules such as proteins. They are used as non-denaturing solvents in the process of protein purification and is especially useful in purifying membrane proteins, which are often sparingly soluble or insoluble in aqueous solution due to their native hydrophobicity. CHAPS can also be used in conjunction with nonionic detergents. CHAPSO has the same basic chemical structure as CHAPS, but has an additional hydroxyl functional group.

Both detergents have low light absorbance in the ultraviolet region of the electromagnetic spectrum, which is useful for laboratory workers monitoring ongoing chemical reactions or protein-protein binding with UV/Vis spectroscopy.

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